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2015-03-09 · Alexa Fluor® 488 A37570* A20000 A20100 A37563* Alexa Fluor in nm, conjugated to an IgG antibody. † Extinction coefficient at Molar Extinction Coefficients vs. Absorbances for 1% Solutions . Application of a . molar.

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475-488Artikel i tidskrift (Refereegranskat) Published 123, 124312 (2005)], the C_6 dipole-dipole dispersion coefficients for the metal cluster-to-cluster and  av M Blomqvist · 2005 · Citerat av 7 — for the change in absorption coefficient with applied electric field. The induced Pb5Ge3O11. 488. 4.8 (293). Aurivillius.

To request additional information and datasets for Alexa Fluor 488, please contact

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Molecular weight: ~914. Excitation laser line: 488 nm. Direct replacement for: Alexa Fluor® 488, DyLight® 488, fluorescein (aka  23 May 2017 the values of the diffusion coefficient D of the AF488 molecules can be at 495 nm with a molar extinction coefficient of 71000. M cm−1  Alexa Fluor 4..

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[emitted Alexa Fluor 488. Extinction coefficient: 70,000.

Af 488 extinction coefficient

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EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Or use a formula that takes into account nearest neighbor effects. An algorithm for this calculation can be found on the web. Just AFDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line.
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Search the world's information, including webpages, images, videos and more. Google has many special features to help you find exactly what you're looking for. Studying the vertical aerosol extinction coefficient by comparing in situ airborne data and elastic backscatter lidar Bernadette Rosati1, Erik Herrmann1, Silvia Bucci2, Federico Fierli2, Francesco Cairo2, Martin Gysel1, Ralf Tillmann3, Johannes Größ4, Gian Paolo Gobbi2, Luca Di Liberto2, Guido Di Donfrancesco5, = 70,000 (NHS-Fluorescein molar extinction coefficient) dilution factor ε protein concentration (M) A of the labeled protein Moles fluor per mole protein fluor max × × = Additional Information Please visit our web site for additional information relating to this product including the following: • Tech Tip #43: Protein Stability and Storage Atto 488-Streptavidin BioReagent, suitable for Degradations, Enzyme-linked immunosorbent assay, Extinction coefficient, Flow cytometry, Fluorescent Answer 2: HiLyte Fluor™ 488 labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbancy maximum for HiLyte 488 ™ at 527 nm and the Beer-Lambert law.

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Direct replacement for: Alexa Fluor® 488, DyLight® 488, fluorescein (aka  23 May 2017 the values of the diffusion coefficient D of the AF488 molecules can be at 495 nm with a molar extinction coefficient of 71000. M cm−1  Alexa Fluor 4.. Alexa Fluor 4.. Alexa Fluor 5. If you do not have accurate extinction coefficients and quantum yields, please make the measurements or arrange  Fluor 555 dye was similar to the Cy3 dye, and the Alexa Fluor 647 dye was Extinction coefficients for all unconjugated dyes in methanol length of 488 nm. Alexa Fluor 488 is a very good choice for flow cytometry.

The molar extinction coefficient (ε) for DyLight 488 is 70,000 cm-1M-1. To request additional information and datasets for DyLight 488, please contact Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH2O or PBS. Need to determine the extinction coefficient of Alexa Fluor® dyes for your experiment? Click the links below to get the data! Alexa Fluor 350: Se hela listan på molar and mass extinction coefficients are listed below: • ε molar is the molar extinction coefficient or molar absorptivity of the protein, expressed as M–1 cm–1 • ε 1% is the mass extinction coefficient or the percent solution extinction coefficient (absorbance values at 280 nm) for a 1% or 10 mg/mL solution of a reference The extinction coefficient (ε) of Alexa Fluor 430 isEasily find your required extinction coefficient (ε) with AAT Bioquest's Extinction Coefficient Database. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. Alexa Fluor 488 spectrum - Alexa Fluor 488 is a fluo excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds.